5-Hydroxymethylfurfural in honey

• Column:
  Synergi Hydro-RP 250х4.6 mm 4 µm
• Column Guard:
  SecurityGuard C18 Aq 4x3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  0.005 mol / dm³ sodium n-heptyl sulfonate in acetonitrile-water solution (10:90)
• Flow rate:
  0 , 8 cm<SUP>3</SUP> / min
• Column temperature:
  room
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  & lamda; 280 nm
• Object of study:
  Honey and honey-containing products
• Substance groups:
  Poisons, toxicants, carcinogens
• Sample:
  natural honey sample 1 , 17 mg / kg
• Methodology:
  Methods for measuring the mass fraction of 5-hydroxymethylfurfural in juices, processed fruits and vegetables, honey and honey-containing products by high performance liquid chromatography