Aflatoxins B1, B2, G1, G2 in a cake

• Column:
  Synergi Hydro-RP 250х4.6 mm 4 µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  n-butanol / acetonitrile / water / acetic acid (8:14:76:1)
• Flow rate:
  0.9 ml / min
• Column temperature:
  50 °С
• Sample volume:
  20 µl
• Detector:
  Fluorimetric detector
• Detection parameters:
  RFU 0.005, Ex-365nm, Em-400-460nm
• Object of study:
  Confectionery
• Substance groups:
  Mycotoxins
• Sample:
  Eclair cake sample with standard addition mixtures of aflatoxins
• Methodology:
  Methods for measuring the mass fraction of aflatoxins B1, B2, G1 and G2 in food by high performance liquid chromatography.