Benz(a)pyrene in a soil sample

• Column:
  Luna C18(2) 150х3.0 mm 3 µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  acetonitrile / water (80:20)
• Flow rate:
  0.3 ml / min
• Column temperature:
  20 °С
• Sample volume:
  10 µl
• Detector:
  Fluorimetric detector
• Detection parameters:
  RFU 0.01 , Ex-365nm, Em-400-460nm
• Object of study:
  Soil (ground)
• Substance groups:
  Polyaromatic hydrocarbons (PAHs)
• Methodology:
  Methods for measuring the mass fraction of benz(a)pyrene in soils, grounds and sewage sludge by high performance liquid chromatography