Benz(a)pyrene in raw smoked fish

• Column:
  Luna C18(2) 150х3.0 mm 3 µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  acetonitrile / water (75:25)
• Flow rate:
  0.3 ml / min
• Column temperature:
  50 °С
• Sample volume:
  10 µl
• Detector:
  Fluorimetric detector
• Detection parameters:
  RFU 0.001, Ex-365nm, Em-400-460nm
• Substance groups:
  Polyaromatic hydrocarbons (PAHs)
• Sample:
  raw smoked fish sample
• Methodology:
  Methods for measuring the mass fraction of benz(a)pyrene in food products, food raw materials, food additives by high performance liquid chromatography