Caffeine in Coca-Cola

• Column:
  Luna C18(2) 250х4.6 mm 5 µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  acetonitrile / water (22:78) + 0.5% phosphoric acid (H₃ PO_{4 )}
• Flow rate:
  1.0 ml / min
• Column temperature:
  20 ° C
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 230 nm
• Object of study:
  Soft drinks
• Substance groups:
  Alkaloids
• Sample:
  Coca-Cola