Carbohydrates in instant coffee

• Column:
  Rezex RPM-Monosaccharide 300х7.8 mm 8 µm
• Column Guard:
  SecurityGuard Carbo-Pb2+ 4х3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  water
• Flow rate:
  0.5 ml / min
• Column temperature:
  85 °С
• Sample volume:
  20 µl
• Detector:
  Refractometric detector
• Object of study:
  Coffee
• Substance groups:
  Carbohydrates
• Sample:
  instant coffee solution
• Methodology:
  Methods for measuring the mass fraction of carbohydrates in coffee soluble by high performance liquid chromatography