Deoxynivalenol in flour

• Column:
  Synergi Hydro-RP 250х4.6 mm 4 µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  20 mM solution of potassium dihydrogen phosphate (KH₂ PO₄) in acetonitrile / distilled water mixture (1:9)
• Flow rate:
  0.9 ml / min
• Column temperature:
  50 °С
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 222 nm
• Object of study:
  Grain and its processing products
• Substance groups:
  Mycotoxins
• Sample:
  flour sample with additive deoxynivalenol standard solution
• Methodology:
  Methods for measuring the mass fraction of deoxynivalenol in food by high performance liquid chromatography