Fat-soluble vitamins in Matrenkino spread

• Column:
  Luna C18(2) 250х4.6 mm 5 µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Gradient
• Mobile phase:
  
  A: acetonitrile
  B: dichloromethane
• Flow rate:
  1.0 ml / min
• Column temperature:
  20 °С
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  spectrophotometric when changing the wavelength of the light source during analysis
  r n0 min - wavelength 436 nm, 10 min - wavelength 280 nm, 27 min - wavelength 436 nm
• Object of study:
  Dairy products, butter, cheese
• Substance groups:
  Vitamins
• Sample:
  spread "Matrenkino"
• Methodology:
  Methods for measuring the mass fraction of vitamins A, E and D3 in food products, food raw materials, feedstuffs, premixes, dietary supplements and vitamin concentrates by high performance liquid chromatography