Melamine in cream

• Column:
  Synergi Hydro-RP 250х4.6 mm 4 µm
• Column Guard:
  SecurityGuard C18 Aq 4x3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  solution of sodium n-heptyl sulfonate 10 mmol / dm³ in 1% aqueous phosphoric acid - acetonitrile (91: 9)
• Flow rate:
  0.9 cm<SUP>3</SUP> / min
• Column temperature:
  room
• Sample volume:
  20 ml
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 234 nm
• Object of study:
  Dairy products, butter, cheese
• Substance groups:
  Poisons, toxicants, carcinogens
• Sample:
  10% cream with the addition of melamine at the level of 1.0 MPC
• Methodology:
  Methods for measuring the mass fraction of melamine in milk, powdered milk mixtures, cream, sour cream, yoghurts and sour milk products by high performance liquid chromatography