Phenobarbital in Valocordin preparation

• Column:
  Luna C18(2) 150х4.6 mm 5µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Gradient
• Mobile phase:
  
  A: 10% acetonitrile solution + 0.05 M phosphate (pH 6.8)
  B: < / B> 90% acetonitrile solution + H₃ PO₄ (pH 2.0–3.0)
• Flow rate:
  1.0 ml / min
• Column temperature:
  20 °С
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 240 nm
• Object of study:
  Pharmaceuticals
• Substance groups:
  Barbiturates
• Sample: