Serum fatty acids

• Column:
  Luna C18(2) 150х2.0 mm 5 µm
• Column Guard:
  SecurityGuard C18 4х2.0 mm
• Separation mode:
  Gradient
• Mobile phase:
  
  A: 0.005% trifluoroacetic acid in deionized water
  B: acetonitrile
• Flow rate:
  0.3 ml / min
• Column temperature:
  50 °C
• Sample volume:
  20 µl
• Detector:
  Low temperature evaporative light scattering detector
• Detection parameters:
  evaporator tube temperature 30 °C, gain - 9, outlet gas pressure - 3 bar
• Object of study:
  Body fluids
• Substance groups:
  Fatty acids
• Sample:
  donor blood serum