Standard mixture of aflatoxins B1, B2, G1, G2
Components:
- Aflatoxin G2
- Aflatoxin G1 after derivatization with iodine J2
- Aflatoxin B2
- Aflatoxin B1 after derivatization with iodine J2
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Column:
Synergi Hydro-RP 250х4.6 mm 4 µm
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Column Guard:
SecurityGuard C18 4х3.0 mm
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Separation mode:
Isocratic
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Mobile phase:
n-butanol / acetonitrile / water / acetic acid (8:14:76:1)
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Flow rate:
0.9 ml / min
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Column temperature:
50 °С
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Sample volume:
20 µl
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Detector:
Fluorimetric detector
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Detection parameters:
RFU 0.005, Ex-365nm, Em-400-460nm
- Object of study:
- Substance groups:
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Sample:
Mix of aflatoxin standards B1, B2, G1, G2 after precolumn derivatization
- Methodology: