Standard mixture of fat-soluble vitamins 2

• Column:
  Synergi Fusion-RP 250х4.6 mm 4 µm
• Column Guard:
  SecurityGuard Fusion-RP 4х3.0 mm
• Separation mode:
  Gradient
• Mobile phase:
  
  A: 1% H₃ PO₄ in water
  B: acetonitrile
• Flow rate:
  1.2 ml / min
• Column temperature:
  20 ° C
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 280 nm
• Object of study:
  Standard mixtures
• Substance groups:
  Antioxidants, Vitamins
• Sample:
  standard mixture of vitamins in hexane
• Methodology:
  Methods for measuring the mass fraction of vitamins A, E and D3 in food products, food raw materials, feedstuffs, premixes, dietary supplements and vitamin concentrates by high performance liquid chromatography