Standard mixture of water soluble vitamins 1

• Column:
  Synergi Hydro-RP 150х4.6 mm 4 µm
• Column Guard:
  SecurityGuard C18 Aq 4x3.0 mm
• Separation mode:
  Gradient
• Mobile phase:
  
  A: 1% H₃ PO₄ in water
  B: acetonitrile
• Flow rate:
  0.9 ml / min
• Column temperature:
  20 °С
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 254 nm
• Object of study:
  Standard mixtures
• Substance groups:
  Vitamins
• Sample:
  mixture of standards of water-soluble vitamins B₁, B₂, B₆, B₁₂, C, PP