Standard mixture of water-soluble vitamins 3

• Column:
  Synergi Fusion-RP 250х4.6 mm 4 µm
• Column Guard:
  SecurityGuard Fusion-RP 4х3.0 mm
• Separation mode:
  Gradient
• Mobile phase:
  
  A: 15 mmol sodium hexyl sulfronate + 1% H₃ PO_{4 < / SUB> in water
  B: acetonitrile}
• Flow rate:
  1 ml / min
• Column temperature:
  20 ° C
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 210nm
• Object of study:
  Standard mixtures
• Substance groups:
  Vitamins
• Sample:
  mixture of standards of water-soluble vitamins B₁, B₂, B₅, B₆, C, PP, H