Standard soft drink additive blend

• Column:
  Luna C18(2) 250х4.6 mm 5 µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  Acetonitrile / Water (22:78) + 0.5% Phosphoric Acid (H₃ PO₄)
• Flow rate:
  1.0 ml / min
• Column temperature:
  20 °С
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 230 nm
• Object of study:
  Standard mixtures
• Substance groups:
  Alkaloids, Sweeteners, Preservatives
• Sample:
  standard mixture of additives (aspartame , benzoic acid, caffeine, saccharin, sorbic acid) to soft drinks
• Methodology:
  Methods for measuring the mass concentration of sorbic and benzoic acids in beverages by high performance liquid chromatography