Watersoluble vitamins in Hexavit preparation

• Column:
  Luna C18(2) 250х4.6 mm 5 µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Gradient
• Mobile phase:
  
  A: 0.6% H₃ PO₄ in water
  B: acetonitrile
• Flow rate:
  0.8 ml / min
• Column temperature:
  20 °С
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 285 nm
• Object of study:
  Pharmaceuticals
• Substance groups:
  Vitamins
• Sample:
  Hexavit vitamin preparation