Zearalenone in bread

• Column:
  Synergi Hydro-RP 250х4.6 mm 4 µm
• Column Guard:
  SecurityGuard C18 4х3.0 mm
• Separation mode:
  Isocratic
• Mobile phase:
  acetonitrile / water / concentrated orthophosphoric acid (H₃ PO₄) (60:40:1)
• Flow rate:
  0.9 ml / min
• Column temperature:
  50 °С
• Sample volume:
  20 µl
• Detector:
  Spectrophotometric detector
• Detection parameters:
  wavelength 280 nm
• Object of study:
  Bread and bakery products
• Substance groups:
  Mycotoxins
• Sample:
  sample of bread with the addition of a standard solution zearalenone
• Methodology:
  Methods for performing measurements of the mass fraction of zearalenone in food by high performance liquid chromatography